Culture-Independent Detection and Genotyping of Mycoplasma pneumoniae in Clinical Specimens from Beijing, China.

Culture-Independent Detection and Genotyping of Mycoplasma pneumoniae in Clinical Specimens from Beijing, China.

Blog Article

A duplex real-time PCR assay was designed for simultaneous detection and genotyping of Mycoplasma pneumoniae (M.pneumoniae).The detection/typing performance of this duplex PCR method, targeting specific genes for M.pneumoniae type 1 (mpn 459) and type 2 (mpna 5864), was compared to that of the previously published MpP1 real-time PCR assay and the genotyping method for the adhesin P1 gene (mpn 141).

A total of 1,344 throat swab specimens collected from patients in Beijing, pothys slogan in english China were tested for M.pneumoniae by bacterial culture, MpP1 real-time PCR assay, and our duplex PCR assay, and positive detection rates of 26.9%, 34.4%, and 33.

7%, respectively, were obtained.The duplex PCR method demonstrated high sensitivity and accuracy for detecting and genotyping M.pneumoniae, and significant differences in genotyping ability were observed when compared to the conventional P1 gene-based method.M.

pneumoniae type 1 was the predominate genotype from 2008 to 2012 in Beijing, and a shift from type 1 to type 2 began to occur in 2013.To our knowledge, this is the hbl5266ca first reported incidence of a type shift phenomenon of M.pneumoniae clinical isolates in China.These genotyping results provide important information for understanding recent changes in epidemiological characteristics of M.

pneumoniae in Beijing.